| clone | 5'-sequence | 3'-sequence | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Seq. | length | contig | BestHit | Evalue | description | length | contig | BestHit | Evalue | description | |
| JMFF020C09 | Sequence | 618 | 000106 (14) | gb|KDP22396.1| | 4e-79 | hypothetical protein JCGZ_26227 [Jatropha curcas] | 579 | 000106 (14) | gb|KDP22396.1| | 2e-79 | hypothetical protein JCGZ_26227 [Jatropha curcas] |
| JMFN002D05 | Sequence | 553 | 000106 (14) | gb|KDP22396.1| | 3e-79 | hypothetical protein JCGZ_26227 [Jatropha curcas] | |||||
| JMFN004C11 | Sequence | 592 | 000106 (14) | gb|KDP22396.1| | 3e-79 | hypothetical protein JCGZ_26227 [Jatropha curcas] | |||||
| JMFN010L12 | Sequence | 570 | 000106 (14) | gb|KDP22396.1| | 4e-79 | hypothetical protein JCGZ_26227 [Jatropha curcas] | |||||
| JMFN013G13 | Sequence | 549 | 000106 (14) | gb|KDP22396.1| | 2e-79 | hypothetical protein JCGZ_26227 [Jatropha curcas] | |||||
| JMFN013H08 | Sequence | 581 | 000106 (14) | gb|KDP22396.1| | 3e-79 | hypothetical protein JCGZ_26227 [Jatropha curcas] | |||||
| JMFN013K24 | Sequence | 570 | 000106 (14) | gb|KDP22396.1| | 4e-79 | hypothetical protein JCGZ_26227 [Jatropha curcas] | |||||
| JMFN032C18 | Sequence | 602 | 000106 (14) | gb|KDP22396.1| | 3e-79 | hypothetical protein JCGZ_26227 [Jatropha curcas] | |||||
| JMFN032M02 | Sequence | 577 | 000106 (14) | gb|KDP22396.1| | 3e-79 | hypothetical protein JCGZ_26227 [Jatropha curcas] | |||||
| JMFN033N07 | Sequence | 594 | 000106 (14) | gb|KDP22396.1| | 3e-79 | hypothetical protein JCGZ_26227 [Jatropha curcas] | |||||
| JMFN045E21 | Sequence | 577 | 000106 (14) | gb|KDP22396.1| | 3e-79 | hypothetical protein JCGZ_26227 [Jatropha curcas] | |||||
| JMSF049P01 | Sequence | 611 | 000106 (14) | gb|KDP22396.1| | 4e-79 | hypothetical protein JCGZ_26227 [Jatropha curcas] | 561 | 000106 (14) | gb|KDP22396.1| | 2e-79 | hypothetical protein JCGZ_26227 [Jatropha curcas] |
This contig is upended
. : . : . : . : . : . :
JMFN013H08+ AATTCAA
JMFN032M02+ AATTCAA
JMFN045E21+ AATTCAA
JMFN010L12+ AATTCAA
JMFN013K24+ AATTCAA
JMFN013G13+ AATTCAA
JMFN004C11+ AATTCAA
JMFN032C18+ AATTCAA
____________________________________________________________
consensus AATTCAA
. : . : . : . : . : . :
JMFN013H08+ ACTCAAATAAGATTAATAACAAAAACTATTGTCACATACTGAGTATTTGTTAACTTCAAA
JMFN032M02+ ACTCAAATAAGATTAATAACAAAAACTATTGTCACATACTGAGTATTTGTTAACTTCAAA
JMFN045E21+ ACTCAAATAAGATTAATAACAAAAACTATTGTCACATACTGAGTATTTGTTAACTTCAAA
JMFN010L12+ ACTCAAATAAGATTAATAACAAAAACTATTGTCACATACTGAGTATTTGTTAACTTCAAA
JMFN013K24+ ACTCAAATAAGATTAATAACAAAAACTATTGTCACATACTGAGTATTTGTTAACTTCAAA
JMFN002D05+ CAAAAACTATTGTCACATACTGAGTATTTGTTAACTTCAAA
JMFN013G13+ ACTCAAATAAGATTAATAACAAAAACTATTGTCACATACTGAGTATTTGTTAACTTCAAA
rJMSF049P01- GTATTTGTTAACTTCAAA
JMFN004C11+ ACTCAAATAAGATTAATAACAAAAACTATTGTCACATACTGAGTATTTGTTAACTTCAAA
JMFN032C18+ ACTCAAATAAGATTAATAACAAAAACTATTGTCACATACTGAGTATTTGTTAACTTCAAA
JMFN033N07+ CTCAAATAAGATTAATAACAAAAACTATTGTCACATACTGAGTATTTGTTAACTTCAAA
rJMFF020C09- AAAACTATTGTCACATACTGAGTATTTGTTAACTTCAAA
____________________________________________________________
consensus ACTCAAATAAGATTAATAACAAAAACTATTGTCACATACTGAGTATTTGTTAACTTCAAA
. : . : . : . : . : . :
JMFN013H08+ AATATAATTTGAAAGCTGATGAATCTATCCTGTCAACAATATTTAAACAGATAATAACTT
JMFN032M02+ AATATAATTTGAAAGCTGATGAATCTATCCTGTCAACAATATTTAAACAGATAATAACTT
JMFN045E21+ AATATAATTTGAAAGCTGATGAATCTATCCTGTCAACAATATTTAAACAGATAATAACTT
JMFN010L12+ AATATAATTTGAAAGCTGATGAATCTATCCTGTCAACAATATTTAAACAGATAATAACTT
JMFN013K24+ AATATAATTTGAAAGCTGATGAATCTATCCTGTCAACAATATTTAAACAGATAATAACTT
JMFN002D05+ AATATAATTTGAAAGCTGATGAATCTATCCTGTCAACAATATTTAAACAGATAATAACTT
JMFN013G13+ AATATAATTTGAAAGCTGATGAATCTATCCTGTCAACAATATTTAAACAGATAATAACTT
rJMSF049P01- AATATAATTTGAAAGCTGATGAATCTATCCTGTCAACAATATTTAAACAGATAATAACTT
JMFN004C11+ AATATAATTTGAAAGCTGATGAATCTATCCTGTCAACAATATTTAAACAGATAATAACTT
JMFN032C18+ AATATAATTTGAAAGCTGATGAATCTATCCTGTCAACAATATTTAAACAGATAATAACTT
JMFN033N07+ AATATAATTTGAAAGCTGATGAATCTATCCTGTCAACAATATTTAAACAGATAATAACTT
rJMFF020C09- AATATAATTTGAAAGCTGATGAATCTATCCTGTCAACAATATTTAAACAGATAATAACTT
____________________________________________________________
consensus AATATAATTTGAAAGCTGATGAATCTATCCTGTCAACAATATTTAAACAGATAATAACTT
. : . : . : . : . : . :
JMFN013H08+ CAAAAACACAACTCCGCTAGTGCTTTATAGTTCAAGAATCAGCAATGGTAACCTCAACCT
JMFN032M02+ CAAAAACACAACTCCGCTAGTGCTTTATAGTTCAAGAATCAGCAATGGTAACCTCAACCT
JMFN045E21+ CAAAAACACAACTCCGCTAGTGCTTTATAGTTCAAGAATCAGCAATGGTAACCTCAACCT
JMFN010L12+ CAAAAACACAACTCCGCTAGTGCTTTATAGTTCAAGAATCAGCAATGGTAACCTCAACCT
JMFN013K24+ CAAAAACACAACTCCGCTAGTGCTTTATAGTTCAAGAATCAGCAATGGTAACCTCAACCT
JMFN002D05+ CAAAAACACAACTCCGCTTGTGCTTTATAGTTCAAGAATCAGCAATGGTAACCTCAACCT
JMFN013G13+ CAAAAACACAACTCCGCTAGTGCTTTATAGTTCAAGAATCAGCAATGGTAACCTCAACCT
rJMSF049P01- CAAAAACACAACTCCGCTAGTGCTTTATAGTTCAAGAATCAGCAATGGTAACCTCAACCT
JMSF049P01+ AAGAATCAGCAATGGTAACCTCAACCT
JMFN004C11+ CAAAAACACAACTCCGCTAGTGCTTTATAGTTCAAGAATCAGCAATGGTAACCTCAACCT
JMFN032C18+ CAAAAACACAACTCCGCTAGTGCTTTATAGTTCAAGAATCAGCAATGGTAACCTCAACCT
JMFN033N07+ CAAAAACACAACTCCGCTAGTGCTTTATAGTTCAAGAATCAGCAATGGTAACCTCAACCT
rJMFF020C09- CAAAAACACAACTCCGCTAGTGCTTTATAGTTCAAGAATCAGCAATGGTAACCTCAACCT
JMFF020C09+ AAGAATCAGCAATGGTAACCTCAACCT
____________________________________________________________
consensus CAAAAACACAACTCCGCTAGTGCTTTATAGTTCAAGAATCAGCAATGGTAACCTCAACCT
. : . : . : . : . : . :
JMFN013H08+ CCACCCCAGGCTCAATTGTAATTGAAGTGATCTGCTTCACCACTTCGGGAGAGCTAAATA
JMFN032M02+ CCACCCCAGGCTCAATTGTAATTGAAGTGATCTGCTTCACCACTTCGGGAGAGCTAAATA
JMFN045E21+ CCACCCCAGGCTCAATTGTAATTGAAGTGATCTGCTTCACCACTTCGGGAGAGCTAAATA
JMFN010L12+ CCACCCCAGGCTCAATTGTAATTGAAGTGATCTGCTTCACCACTTCGGGAGAGCTAAATA
JMFN013K24+ CCACCCCAGGCTCAATTGTAATTGAAGTGATCTGCTTCACCACTTCGGGAGAGCTAAATA
JMFN002D05+ CCACCCCAGGCTCAATTGTAATTGAAGTGATCTGCTTCACCACTTCGGGAGAGCTAAATA
JMFN013G13+ CCACCCCAGGCTCAATTGTAATTGAAGTGATCTGCTTCACCACTTCGGGAGAGCTAAATA
rJMSF049P01- CCACCCCAGGCTCAATTGTAATTGAAGTGATCTGCTTCACCACTTCGGGAGAGCTAAATA
JMSF049P01+ CCACCCCAGGCTCAATTGTAATTGAAGTGATCTGCTTCACCACTTCGGGAGAGCTAAATA
JMFN004C11+ CCACCCCAGGCTCAATTGTAATTGAAGTGATCTGCTTCACCACTTCGGGAGAGCTAAATA
JMFN032C18+ CCACCCCAGGCTCAATTGTAATTGAAGTGATCTGCTTCACCACTTCGGGAGAGCTAAATA
JMFN033N07+ CCACCCCAGGCTCAATTGTAATTGAAGTGATCTGCTTCACCACTTCGGGAGAGCTAAATA
rJMFF020C09- CCACCCCAGGCTCAATTGTAATTGAAGTGATCTGCTTCACCACTTCGGGAGAGCTAAATA
JMFF020C09+ CCACCCCAGGCTCAATTGTAATTGAAGTGATCTGCTTCACCACTTCGGGAGAGCTAAATA
____________________________________________________________
consensus CCACCCCAGGCTCAATTGTAATTGAAGTGATCTGCTTCACCACTTCGGGAGAGCTAAATA
. : . : . : . : . : . :
JMFN013H08+ GGTCAATCACACGCTTGTGGACCCGAAGCTCGAACCTATCCCAAGTGTTGGTACCTTCTC
JMFN032M02+ GGTCAATCACACGCTTGTGGACCCGAAGCTCGAACCTATCCCAAGTGTTGGTACCTTCTC
JMFN045E21+ GGTCAATCACACGCTTGTGGACCCGAAGCTCGAACCTATCCCAAGTGTTGGTACCTTCTC
JMFN010L12+ GGTCAATCACACGCTTGTGGACCCGAAGCTCGAACCTATCCCAAGTGTTGGTACCTTCTC
JMFN013K24+ GGTCAATCACACGCTTGTGGACCCGAAGCTCGAACCTATCCCAAGTGTTGGTACCTTCTC
JMFN002D05+ GGTCAATCACACGCTTGTGGACCCGAAGCTCGAACCTATCCCAAGTGTTGGTACCTTCTC
JMFN013G13+ GGTCAATCACACGCTTGTGGACCCGAAGCTCGAACCTATCCCAAGTGTTGGTACCTTCTC
rJMSF049P01- GGTCAATCACACGCTTGTGGACCCGAAGCTCGAACCTATCCCAAGTGTTGGTACCTTCTC
JMSF049P01+ GGTCAATCACACGCTTGTGGACCCGAAGCTCGAACCTATCCCAAGTGTTGGTACCTTCTC
JMFN004C11+ GGTCAATCACACGCTTGTGGACCCGAAGCTCGAACCTATCCCAAGTGTTGGTACCTTCTC
JMFN032C18+ GGTCAATCACACGCTTGTGGACCCGAAGCTCGAACCTATCCCAAGTGTTGGTACCTTCTC
JMFN033N07+ GGTCAATCACACGCTTGTGGACCCGAAGCTCGAACCTATCCCAAGTGTTGGTACCTTCTC
rJMFF020C09- GGTCAATCACACGCTTGTGGACCCGAAGCTCGAACCTATCCCAAGTGTTGGTACCTTCTC
JMFF020C09+ GGTCAATCACACGCTTGTGGACCCGAAGCTCGAACCTATCCCAAGTGTTGGTACCTTCTC
____________________________________________________________
consensus GGTCAATCACACGCTTGTGGACCCGAAGCTCGAACCTATCCCAAGTGTTGGTACCTTCTC
. : . : . : . : . : . :
JMFN013H08+ CACAGGGAGACTTTCTAGTAGTAATATGAAGAACCTTGGTAGGCATCCTAACCGGTCCCT
JMFN032M02+ CACAGGGAGACTTTCTAGTAGTAATATGAAGAACCTTGGTAGGCATCCTAACCGGTCCCT
JMFN045E21+ CACAGGGAGACTTTCTAGTAGTAATATGAAGAACCTTGGTAGGCATCCTAACCGGTCCCT
JMFN010L12+ CACAGGGAGACTTTCTAGTAGTAATATGAAGAACCTTGGTAGGCATCCTAACCGGTCCCT
JMFN013K24+ CACAGGGAGACTTTCTAGTAGTAATATGAAGAACCTTGGTAGGCATCCTAACCGGTCCCT
JMFN002D05+ CACAGGGAGACTTTCTAGTAGTAATATGAAGAACCTTGGTAGGCATCCTAACCGGTCCCT
JMFN013G13+ CACAGGGAGACTTTCTAGTAGTAATATGAAGAACCTTGGTAGGCATCCTAACCGGTCCCT
rJMSF049P01- CACAGGGAGACTTTCTAGTAGTAATATGAAGAACCTTGGTAGGCATCCTAACCGGTCCCT
JMSF049P01+ CACAGGGAGACTTTCTAGTAGTAATATGAAGAACCTTGGTAGGCATCCTAACCGGTCCCT
JMFN004C11+ CACAGGGAGACTTTCTAGTAGTAATATGAAGAACCTTGGTAGGCATCCTAACCGGTCCCT
JMFN032C18+ CACAGGGAGACTTTCTAGTAGTAATATGAAGAACCTTGGTAGGCATCCTAACCGGTCCCT
JMFN033N07+ CACAGGGAGACTTTCTAGTAGTAATATGAAGAACCTTGGTAGGCATCCTAACCGGTCCCT
rJMFF020C09- CACAGGGAGACTTTCTAGTAGTAATATGAAGAACCTTGGTAGGCATCCTAACCGGTCCCT
JMFF020C09+ CACAGGGAGACTTTCTAGTAGTAATATGAAGAACCTTGGTAGGCATCCTAACCGGTCCCT
____________________________________________________________
consensus CACAGGGAGACTTTCTAGTAGTAATATGAAGAACCTTGGTAGGCATCCTAACCGGTCCCT
. : . : . : . : . : . :
JMFN013H08+ TGACCCTGAGTCTCTTGTCCTTGGCACCCCGGACCAAGTCAGCACAAACTTTCTCGAGAT
JMFN032M02+ TGACCCTGAGTCTCTTGTCCTTGGCACCCCGGACCAAGTCAGCACAAACTTTCTCGAGAT
JMFN045E21+ TGACCCTGAGTCTCTTGTCCTTGGCACCCCGGACCAAGTCAGCACAAACTTTCTCGAGAT
JMFN010L12+ TGACCCTGAGTCTCTTGTCCTTGGCACCCCGGACCAAGTCAGCACAAACTTTCTCGAGAT
JMFN013K24+ TGACCCTGAGTCTCTTGTCCTTGGCACCCCGGACCAAGTCAGCACAAACTTTCTCGAGAT
JMFN002D05+ TGACCCTGAGTCTCTTGTCCTTGGCACCCCGGACCAAGTCAGCACAAACTTTCTCGAGAT
JMFN013G13+ TGACCCTGAGTCTCTTGTCCTTGGCACCCCGGACCAAGTCAGCACAAACTTTCTCGAGAT
rJMSF049P01- TGACCCTGAGTCTCTTGTCCTTGGCACCCCGGACCAAGTCAGCACAAACTTTCTCGAGAT
JMSF049P01+ TGACCCTGAGTCTCTTGTCCTTGGCACCCCGGACCAAGTCAGCACAAACTTTCTCGAGAT
JMFN004C11+ TGACCCTGAGTCTCTTGTCCTTGGCACCCCGGACCAAGTCAGCACAAACTTTCTCGAGAT
JMFN032C18+ TGACCCTGAGTCTCTTGTCCTTGGCACCCCGGACCAAGTCAGCACAAACTTTCTCGAGAT
JMFN033N07+ TGACCCTGAGTCTCTTGTCCTTGGCACCCCGGACCAAGTCAGCACAAACTTTCTCGAGAT
rJMFF020C09- TGACCCTGAGTCTCTTGTCCTTGGCACCCCGGACCAAGTCAGCACAAACTTTCTCGAGAT
JMFF020C09+ TGACCCTGAGTCTCTTGTCCTTGGCACCCCGGACCAAGTCAGCACAAACTTTCTCGAGAT
____________________________________________________________
consensus TGACCCTGAGTCTCTTGTCCTTGGCACCCCGGACCAAGTCAGCACAAACTTTCTCGAGAT
. : . : . : . : . : . :
JMFN013H08+ TCTTAACGTTTTTGGAAGAGAGAGTGATTCTGATCTTGTGAATCTGTTCCTGGGGCTCCT
JMFN032M02+ TCTTAACGTTTTTGGAAGAGAGAGTGATTCTGATCTTGTGAATCTGTTCCTGGGGCTCCT
JMFN045E21+ TCTTAACGTTTTTGGAAGAGAGAGTGATTCTGATCTTGTGAATCTGTTCCTGGGGCTCCT
JMFN010L12+ TCTTAACGTTTTTGGAAGAGAGAGTGATTCTGATCTTGTGAATCTGTTCCTGGGGCTCCT
JMFN013K24+ TCTTAACGTTTTTGGAAGAGAGAGTGATTCTGATCTTGTGAATCTGTTCCTGGGGCTCCT
JMFN002D05+ TCTTAACGTTTTTGGAAGAGAGAGTGATTCTGATCTTGTGAATCTGTTCCTGGGGCTCCT
JMFN013G13+ TCTTAACGTTTTTGGAAGAGAGAGTGATTCTGATCTTGTGAATCTGTTCCTGGGGCTCCT
rJMSF049P01- TCTTAACGTTTTTGGAAGAGAGAGTGATTCTGATCTTGTGAATCTGTTCCTGGGGCTCCT
JMSF049P01+ TCTTAACGTTTTTGGAAGAGAGAGTGATTCTGATCTTGTGAATCTGTTCCTGGGGCTCCT
JMFN004C11+ TCTTAACGTTTTTGGAAGAGAGAGTGATTCTGATCTTGTGAATCTGTTCCTGGGGCTCCT
JMFN032C18+ TCTTAACGTTTTTGGAAGAGAGAGTGATTCTGATCTTGTGAATCTGTTCCTGGGGCTCCT
JMFN033N07+ TCTTAACGTTTTTGGAAGAGAGAGTGATTCTGATCTTGTGAATCTGTTCCTGGGGCTCCT
rJMFF020C09- TCTTAACGTTTTTGGAAGAGAGAGTGATTCTGATCTTGTGAATCTGTTCCTGGGGCTCCT
JMFF020C09+ TCTTAACGTTTTTGGAAGAGAGAGTGATTCTGATCTTGTGAATCTGTTCCTGGGGCTCCT
____________________________________________________________
consensus TCTTAACGTTTTTGGAAGAGAGAGTGATTCTGATCTTGTGAATCTGTTCCTGGGGCTCCT
. : . : . : . : . : . :
JMFN013H08+ CCAGCCCAGCCTTTGCCGGCTTCACCGCTTGATACGCCATTTTTTCACTTCGCTTTCGGA
JMFN032M02+ CCAGCCCAGCCTTTGCCGGCTTCACCGCTTGATACGCCATTTTTTCACTTCGCTTTCGGA
JMFN045E21+ CCAGCCCAGCCTTTGCCGGCTTCACCGCTTGATACGCCATTTTTTCACTTCGCTTTCGGA
JMFN010L12+ CCAGCCCAGCCTTTGCCGGCTTCACCGCTTGATACGCCATTTTTTCACTTCGCTTTCGGA
JMFN013K24+ CCAGCCCAGCCTTTGCCGGCTTCACCGCTTGATACGCCATTTTTTCACTTCGCTTTCGGA
JMFN002D05+ CCAGCCCAGCCTTTGCCGGCTTCACCGCTTGATACGCCATTTTTTCACTTCGCTTTCGGA
JMFN013G13+ CCAGCCCAGCCTTTGCCGGCTTCACCGCTTGATACGCCATTTTTTCACTTCGCTTTCGGA
rJMSF049P01- CCAGCCCAGCCTTTGCCGGCTTCACCGCTTGATACGCCATTTTTTCACTTCGCTTTCGGA
JMSF049P01+ CCAGCCCAGCCTTTGCCGGCTTCACCGCTTGATACGCCATTTTTTCACTTCGCTTTCGGA
JMFN004C11+ CCAGCCCAGCCTTTGCCGGCTTCACCGCTTGATACGCCATTTTTTCACTTCGCTTTCGGA
JMFN032C18+ CCAGCCCAGCCTTTGCCGGCTTCACCGCTTGATACGCCATTTTTTCACTTCGCTTTCGGA
JMFN033N07+ CCAGCCCAGCCTTTGCCGGCTTCACCGCTTGATACGCCATTTTTTCACTTCGCTTTCGGA
rJMFF020C09- CCAGCCCAGCCTTTGCCGGCTTCACCGCTTGATACGCCATTTTTTCACTTCGCTTTCGGA
JMFF020C09+ CCAGCCCAGCCTTTGCCGGCTTCACCGCTTGATACGCCATTTTTTCACTTCGCTTTCGGA
____________________________________________________________
consensus CCAGCCCAGCCTTTGCCGGCTTCACCGCTTGATACGCCATTTTTTCACTTCGCTTTCGGA
. : . : . : . : . : . :
JMFN013H08+ GAGGCGGAGAGCGAACTGGCGAAGAAGTAAGGGA
JMFN032M02+ GAGGCGGAGAGCGAACTGGCGAAGAAGTAA
JMFN045E21+ GAGGCGGAGAGCGAACTGGCGAAGAAGTAA
JMFN010L12+ GAGGCGGAGAGCGAACTGGCGAA
JMFN013K24+ GAGGCGGAGAGCGAACTGGCGAA
JMFN002D05+ GAGGCGGAGAGCGAACTGGCGAAGAAGTAAGG
JMFN013G13+ GA
rJMSF049P01- GAGGCGGAGAGCGAACTGGCGAAGAAGTAAGGGAGATGCGGCT
JMSF049P01+ GAGGCGGAGAGCGAACTGGCGAAGAAGTAAGGGAGATGCGGCT
JMFN004C11+ GAGGCGGAGAGCGAACTGGCGAAGAAGTAAGGGAGATGCGGCTAA
JMFN032C18+ GAGGCGGAGAGCGAACTGGCGAAGAAGTAAGGGAGATGCGGCTAACTGGAAGAAG
JMFN033N07+ GAGGCGGAGAGCGAACTGGCGAAGAAGTAAGGGAGATGCGGCTAACTGGAAGAAG
rJMFF020C09- GAGGCGGAGAGCGAACTGGCGAAGAAGTAAGGGAGATGCGGCTAACTGGAAGAAG
JMFF020C09+ GAGGCGGAGAGCGAACTGGCGAAGAAGTAAGGGAGATGCGGCTAACTGGAAGAAGAAGAC
____________________________________________________________
consensus GAGGCGGAGAGCGAACTGGCGAAGAAGTAAGGGAGATGCGGCTAACTGGAAGAAGAAGAC